Yeast surface display of full‐length human microtubule‐associated protein tau
نویسندگان
چکیده
منابع مشابه
Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display.
Reliable and robust systems for engineering functional major histocompatibility complex class II (MHCII) proteins have proved elusive. Availability of such systems would enable the engineering of peptide-MHCII (pMHCII) complexes for therapeutic and diagnostic applications. In this paper, we have developed a system based on insect cell surface display that allows functional expression of heterod...
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Yeast surface display (YSD) is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. Since first described in 1997 by Boder and Wittrup, YSD has been employed successfully in engineering a number of antibodies, as well as T-cell receptors. A recently reported large nonimmune single chain antibody library serves as a good starting poin...
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The described protocols enable thorough screening of polypeptide libraries with high confidence in the isolation of improved clones. It should be emphasized that the protocols have been fashioned for thoroughness, rather than speed. With library plasmid DNA in hand, the time to plated candidate yeast display mutants is typically 2-3 weeks. Each of the experimental approaches required for this m...
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ژورنال
عنوان ژورنال: Biotechnology Progress
سال: 2019
ISSN: 8756-7938,1520-6033
DOI: 10.1002/btpr.2920